Detection of Bovine Herpesvirus 1 from an Outbreak of Infectious Bovine Rhinotracheitis
نویسندگان
چکیده
Clinical symptoms from the respiratory tract were observed in cattle after the introduction of pregnant heifers into the dairy herd. Sera and nasal swabs from all animals and tissue samples from two dead animals were tested for BHV1. Specific antibodies against BHV1 were found in serum samples of 24 animals. Only one sample reacted doubtfully in gB ELISA. The virus was isolated only from nasal swabs and lungs collected from 2 weeks old calf. The remaining samples were negative in virus isolation test. PCR with external primers detected the presence of BHV1 in 11 nasal swabs and in lung and liver samples of 2 weeks old calf. In nested PCR almost all tested samples were positive. Restriction enzyme analysis confirmed the specificity of amplification. Results of laboratory diagnosis revealed that introduction of newly purchased animals into the herd initiated the outbreak of disease caused by BHV1.
منابع مشابه
Detection of antibody against infectious bovine rhinotracheitis glycoprotein gE in aborted cattle in Mashhad, Iran
Infectious Bovine Rhinotracheitis is a highly contagious disease caused by the bovine herpes virus-1 (BoHV-1), resulting in significant losses to livestock around the world. BoHV-1 is a major pathogen of cattle, primarily associated with respiratory/genital tract infections and abortion. In the present study, we determined the presence of antibodies in 120 serum samples of cattle with the histo...
متن کاملPrevalence of infectious bovine rhinotracheitis in southern India.
The authors describe a serological survey on the prevalence of infectious bovine rhinotracheitis (IBR) among cattle and buffalo (Bubalus bubalis) in three southern states of India. A local isolate of bovine herpesvirus 1 (BHV-1) from an outbreak of the respiratory form of IBR was used as a source of virus antigen in avidin-biotin enzyme-linked immunosorbent assay (ELISA). The overall prevalence...
متن کاملSeroepidemological Survey for Antibodies against Infectious Bovine Rhinotracheitis and Bovine Herpes 4 Viruses among Cattle in Different Provinces of Iran.
متن کامل
Isolation of Infectious Bovine Rhinotracheitis Virus in Cell Culture: (a Case Study)
Infectious bovine rhinotracheitis is an important disease of cattle with serious economic implications. The etiology is bovine herpes virus type-1 which leads to clinical to latent infections and abortions. In this study, an aborted bovine fetus was suspected of IBR infection. The virus was grown in cell line (MDBK) and cytopathic effect observed. CPE began at 12 hr post infection of cells and ...
متن کاملAn adventitious viral contaminant in commercially supplied A549 cells: identification of infectious bovine rhinotracheitis virus and its impact on diagnosis of infection in clinical specimens.
The isolation and identification of an adventitious viral agent, infectious bovine rhinotracheitis virus, in one lot of A549 cells from a commercial supplier is described in this report. The presence of infectious bovine rhinotracheitis virus in A549 cells was unexpected and has caused problems in the diagnosis of infections in clinical specimens in three laboratories.
متن کامل